1Y7A
Structure of D153H/K328W E. coli alkaline phosphatase in presence of cobalt at 1.77 A resolution
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | NSLS BEAMLINE X26C |
| Synchrotron site | NSLS |
| Beamline | X26C |
| Temperature [K] | 93 |
| Detector technology | CCD |
| Collection date | 2003-11-23 |
| Detector | ADSC QUANTUM 4 |
| Spacegroup name | I 2 2 2 |
| Unit cell lengths | 76.208, 164.564, 192.636 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 30.000 - 1.770 |
| R-factor | 0.205 |
| Rwork | 0.205 |
| R-free | 0.23800 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1ed8 |
| Data reduction software | HKL-2000 |
| Data scaling software | SCALEPACK |
| Phasing software | AMoRE |
| Refinement software | CNS |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 30.000 | 1.830 |
| High resolution limit [Å] | 1.770 | 1.770 |
| Rmerge | 0.085 | 0.239 |
| Number of reflections | 117928 | |
| <I/σ(I)> | 26.6 | |
| Completeness [%] | 94.5 | 89.6 |
| Redundancy | 12 | 10.3 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 9.5 | 293 | 10 mM cobalt chloride, 2.2 M ammonium sulfate, pH 9.5, VAPOR DIFFUSION, HANGING DROP, temperature 293K |
