1Y71
X-ray crystal structure of kinase-associated protein B from Bacillus cereus
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 19-BM |
| Synchrotron site | APS |
| Beamline | 19-BM |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2004-12-02 |
| Detector | SBC-3 |
| Wavelength(s) | 0.979456 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 27.197, 99.403, 101.755 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 40.000 - 1.950 |
| R-factor | 0.216 |
| Rwork | 0.214 |
| R-free | 0.26500 |
| Structure solution method | SAD |
| RMSD bond length | 0.016 |
| RMSD bond angle | 1.396 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | SHELXD |
| Refinement software | REFMAC |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 40.000 | 2.000 |
| High resolution limit [Å] | 1.950 | 1.950 |
| Rmerge | 0.094 | 0.527 |
| Number of reflections | 20380 | |
| <I/σ(I)> | 19.7 | 1.46 |
| Completeness [%] | 97.2 | 75.7 |
| Redundancy | 7.9 | 4.2 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 4.5 | 287 | 100 mM Sodium Acetate, 20% PEG 3350, pH 4.5, VAPOR DIFFUSION, SITTING DROP, temperature 287K |
