1Y6X

The 1.25 A resolution structure of phosphoribosyl-ATP pyrophosphohydrolase from Mycobacterium tuberculosis

Experimental procedure

Experimental method	MAD
Source type	SYNCHROTRON
Source details	APS BEAMLINE 14-BM-D
Synchrotron site	APS
Beamline	14-BM-D
Temperature [K]	100
Detector technology	CCD
Collection date	2002-03-02
Detector	ADSC QUANTUM 4
Wavelength(s)	1.0037, 0.9802,0.9799, 0.9572
Spacegroup name	I 2 2 2
Unit cell lengths	37.925, 62.597, 67.051
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	45.640 - 1.250
Rwork	0.183
R-free	0.20747
Structure solution method	MAD
RMSD bond length	0.006
RMSD bond angle	0.985
Data reduction software	MOSFLM
Data scaling software	CCP4 ((SCALA))
Phasing software	SHELXD
Refinement software	REFMAC (5.2.0005)

Data quality characteristics

	Overall
Low resolution limit [Å]	45.640
High resolution limit [Å]	1.100
Number of reflections	24294
Completeness [%]	100.0

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	6.5	291	sodium citrate, sodium cacodylate, pH 6.5, VAPOR DIFFUSION, HANGING DROP, temperature 291.0K