1Y6M

Crystal structure of Epstein-Barr virus IL-10 complexed with the soluble IL-10R1 chain

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRL BEAMLINE BL9-2
Synchrotron site	SSRL
Beamline	BL9-2
Temperature [K]	100
Detector technology	CCD
Detector	ADSC QUANTUM 4
Wavelength(s)	0.9184
Spacegroup name	P 32 1 2
Unit cell lengths	47.121, 47.121, 300.708
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	25.000 - 2.800
R-factor	0.264
Rwork	0.261
R-free	0.31500
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.009
RMSD bond angle	1.510
Data reduction software	DENZO
Data scaling software	SCALEPACK
Phasing software	CNS
Refinement software	CNS

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	25.000	2.900
High resolution limit [Å]	2.800	2.800
Rmerge	0.049	0.173
Number of reflections	9518
Completeness [%]	96.4	85.3
Redundancy	9

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	6.2	298	PEG 6000, magnesium chloride, ADA, pH 6.2, VAPOR DIFFUSION, HANGING DROP, temperature 298K