1Y66

Dioxane contributes to the altered conformation and oligomerization state of a designed engrailed homeodomain variant

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	ROTATING ANODE
Source details	RIGAKU RU300
Temperature [K]	100
Detector technology	IMAGE PLATE
Collection date	2003-12-21
Detector	RIGAKU RAXIS IV
Wavelength(s)	1.5418
Spacegroup name	P 21 21 21
Unit cell lengths	50.712, 52.646, 82.182
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	24.300 - 1.650
R-factor	0.18951
Rwork	0.187
R-free	0.22786
Structure solution method	SAD
RMSD bond length	0.017
RMSD bond angle	1.527
Data reduction software	DENZO
Data scaling software	SCALEPACK
Phasing software	MLPHARE
Refinement software	REFMAC (5.2.0005)

Data quality characteristics

	Overall
High resolution limit [Å]	1.650
Number of reflections	27056

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	5.5	293	dioxane, sodium citrate, MES, PEG 400, Cadmium Chloride, pH 5.5, VAPOR DIFFUSION, SITTING DROP, temperature 293K
1	VAPOR DIFFUSION, SITTING DROP	5.5	293	dioxane, sodium citrate, MES, PEG 400, Cadmium Chloride, pH 5.5, VAPOR DIFFUSION, SITTING DROP, temperature 293K