1Y64
Bni1p Formin Homology 2 Domain complexed with ATP-actin
Experimental procedure
| Experimental method | MAD |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 19-ID |
| Synchrotron site | APS |
| Beamline | 19-ID |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2004-02-20 |
| Detector | SBC-2 |
| Wavelength(s) | 0.99987, 0.9797, 0.97981, 0.96411 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 231.997, 56.229, 100.945 |
| Unit cell angles | 90.00, 107.70, 90.00 |
Refinement procedure
| Resolution | 30.000 - 3.050 |
| R-factor | 0.313 |
| Rwork | 0.289 |
| R-free | 0.31300 |
| Structure solution method | MAD |
| RMSD bond length | 0.010 |
| RMSD bond angle | 1.780 |
| Data reduction software | HKL-2000 |
| Data scaling software | SCALEPACK |
| Phasing software | MLPHARE |
| Refinement software | CNS |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 31.780 | 3.080 |
| High resolution limit [Å] | 3.050 | 3.050 |
| Rmerge | 0.046 | 0.391 |
| Number of reflections | 26458 | |
| <I/σ(I)> | 24.4 | 1.8 |
| Completeness [%] | 98.4 | 90.8 |
| Redundancy | 2 | 1.8 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7.6 | 298 | potassium bromide, hepes, DTT, Tris, ATP, calcium chloride, ethylene glycol, pH 7.6, VAPOR DIFFUSION, HANGING DROP, temperature 298K |
