1Y5V
tRNA-Guanine Transglycosylase (TGT) in complex with 6-Amino-4-(2-phenylethyl)-1,7-dihydro-8H-imidazo[4,5-g]quinazolin-8-one
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU RU300 |
| Temperature [K] | 100 |
| Detector technology | IMAGE PLATE |
| Collection date | 2003-03-12 |
| Detector | RIGAKU RAXIS IV++ |
| Wavelength(s) | 1.5418 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 90.590, 64.930, 70.120 |
| Unit cell angles | 90.00, 95.83, 90.00 |
Refinement procedure
| Resolution | 10.000 - 1.580 |
| R-factor | 0.1699 |
| Rwork | 0.170 |
| R-free | 0.21230 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1p0d |
| RMSD bond length | 0.009 |
| RMSD bond angle | 0.028 |
| Data reduction software | CrystalClear ((MSC/RIGAKU)) |
| Data scaling software | HKL-2000 |
| Phasing software | CNS |
| Refinement software | SHELXL-97 |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 20.000 | 1.610 |
| High resolution limit [Å] | 1.580 | 1.580 |
| Number of reflections | 53824 | |
| <I/σ(I)> | 12.4 | 1.7 |
| Completeness [%] | 97.1 | 78.4 |
| Redundancy | 2.2 | 1.6 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 5.5 | 291 | PEG 8000, MES, DMSO, DTT, pH 5.5, VAPOR DIFFUSION, HANGING DROP, temperature 291K |
