1Y5H
Crystal structure of truncated Se-Met Hypoxic Response Protein I (HRPI)
Experimental procedure
| Experimental method | MAD |
| Source type | SYNCHROTRON |
| Source details | ALS BEAMLINE 5.0.2 |
| Synchrotron site | ALS |
| Beamline | 5.0.2 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2004-07-03 |
| Detector | ADSC QUANTUM 315 |
| Wavelength(s) | 0.9793, 0.9796, 0.9537 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 77.452, 41.064, 78.395 |
| Unit cell angles | 90.00, 108.41, 90.00 |
Refinement procedure
| Resolution | 38.060 - 1.500 |
| Rwork | 0.222 |
| R-free | 0.23300 |
| Structure solution method | MAD |
| RMSD bond length | 0.004 |
| RMSD bond angle | 1.200 |
| Data reduction software | HKL-2000 |
| Data scaling software | SCALEPACK |
| Phasing software | SHELXD |
| Refinement software | CNS (1.1) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 1.550 |
| High resolution limit [Å] | 1.500 | 1.500 |
| Number of reflections | 66279 | |
| <I/σ(I)> | 20.97 | 1.77 |
| Completeness [%] | 92.4 | 63.7 |
| Redundancy | 6.2 | 3.2 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 9 | 291 | ammonium sulphate, boric acid/KOH, pH 9.0, VAPOR DIFFUSION, SITTING DROP, temperature 291K |
