1Y5E
Crystal structure of Molybdenum cofactor biosynthesis protein B
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 19-BM |
| Synchrotron site | APS |
| Beamline | 19-BM |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2004-11-04 |
| Detector | CUSTOM-MADE |
| Wavelength(s) | 0.97943 |
| Spacegroup name | P 31 2 1 |
| Unit cell lengths | 122.952, 122.952, 74.536 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 47.430 - 1.900 |
| R-factor | 0.188 |
| Rwork | 0.188 |
| R-free | 0.21500 |
| Structure solution method | SAD |
| RMSD bond length | 0.005 |
| RMSD bond angle | 1.300 |
| Data reduction software | HKL-2000 |
| Data scaling software | SCALEPACK |
| Phasing software | HKL-3000 |
| Refinement software | CNS (1.1) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 1.970 |
| High resolution limit [Å] | 1.900 | 1.900 |
| Rmerge | 0.130 | 0.762 |
| Number of reflections | 99009 | |
| <I/σ(I)> | 18.2 | 1.744 |
| Completeness [%] | 99.2 | 94.1 |
| Redundancy | 11.1 | 5.7 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 8 | 293 | MPD, imidazole, MgCl2, pH 8.0, VAPOR DIFFUSION, SITTING DROP, temperature 293K |
