1Y52

structure of insect cell (Baculovirus) expressed AVR4 (C122S)-biotin complex

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ESRF BEAMLINE ID14-2
Synchrotron site	ESRF
Beamline	ID14-2
Temperature [K]	100
Detector technology	CCD
Collection date	2003-06-15
Detector	ADSC QUANTUM 4
Wavelength(s)	0.933
Spacegroup name	P 41 21 2
Unit cell lengths	80.963, 80.963, 140.698
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	38.920 - 1.700
R-factor	0.19332
Rwork	0.193
R-free	0.20038
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.014
RMSD bond angle	1.650
Data reduction software	DENZO
Data scaling software	CCP4 ((SCALA))
Phasing software	AMoRE
Refinement software	REFMAC (5.1.24)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	40.000	1.730
High resolution limit [Å]	1.700	1.700
Number of reflections	51814
Completeness [%]	99.5	99.9
Redundancy	5.5

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	4.6	298	2.0M ammonium sulfate,0.1M sodium acetate, pH 4.6, VAPOR DIFFUSION, HANGING DROP, temperature 298K