1Y50
X-ray crystal structure of Bacillus stearothermophilus Histidine phosphocarrier protein (Hpr) F29W mutant domain_swapped dimer
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 19-ID |
| Synchrotron site | APS |
| Beamline | 19-ID |
| Detector technology | CCD |
| Spacegroup name | I 2 2 2 |
| Unit cell lengths | 34.861, 63.454, 82.879 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 25.000 - 2.000 |
| R-factor | 0.21931 |
| Rwork | 0.215 |
| R-free | 0.29786 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | wild-type BstHpr |
| RMSD bond length | 0.011 |
| RMSD bond angle | 1.388 |
| Data reduction software | HKL-2000 |
| Data scaling software | SCALEPACK |
| Phasing software | MOLREP |
| Refinement software | REFMAC (5.1.24) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 2.070 |
| High resolution limit [Å] | 2.000 | 2.000 |
| Number of reflections | 6174 | |
| <I/σ(I)> | 31.6 | 2 |
| Completeness [%] | 94.4 | 70.9 |
| Redundancy | 5.2 | 2.5 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 6.3 | 291 | 68-74 % ammonium sulfate, pH 6.3, VAPOR DIFFUSION, HANGING DROP, temperature 291.0K |
