1Y4K
Lipoxygenase-1 (Soybean) at 100K, N694G Mutant
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 19-BM |
| Synchrotron site | APS |
| Beamline | 19-BM |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2003-10-12 |
| Detector | SBC-3 |
| Wavelength(s) | 1.03106 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 93.884, 92.835, 49.435 |
| Unit cell angles | 90.00, 90.66, 90.00 |
Refinement procedure
| Resolution | 33.840 - 1.950 |
| R-factor | 0.18257 |
| Rwork | 0.180 |
| R-free | 0.23243 |
| Structure solution method | ISOMORPHOUS REPLACEMENT |
| Starting model (for MR) | 1f8n |
| RMSD bond length | 0.019 |
| RMSD bond angle | 1.678 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | CNS |
| Refinement software | REFMAC (5.2.0005) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 2.020 |
| High resolution limit [Å] | 1.950 | 1.950 |
| Rmerge | 0.044 | 0.333 |
| Number of reflections | 60260 | |
| <I/σ(I)> | 21.3339 | 3.077 |
| Completeness [%] | 95.9 | 91 |
| Redundancy | 2.8 | 2.7 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 5.2 | 293 | 50 mM Na acetate pH 5.2; 14% w/v PEG 3350, pH 5.20, VAPOR DIFFUSION, SITTING DROP, temperature 293K |
