1Y4I
Crystal structure of Citrobacter Freundii L-methionine-lyase
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | EMBL/DESY, HAMBURG BEAMLINE BW7A |
| Synchrotron site | EMBL/DESY, HAMBURG |
| Beamline | BW7A |
| Temperature [K] | 110 |
| Detector technology | CCD |
| Collection date | 2004-06-11 |
| Detector | MARRESEARCH |
| Wavelength(s) | 0.979 |
| Spacegroup name | I 2 2 2 |
| Unit cell lengths | 56.350, 121.830, 127.160 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 18.660 - 1.900 |
| R-factor | 0.206 |
| Rwork | 0.206 |
| R-free | 0.20900 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1gc0 |
| RMSD bond length | 0.022 |
| RMSD bond angle | 1.600 |
| Data scaling software | XDS |
| Phasing software | AMoRE |
| Refinement software | CNS (1.1) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 20.000 | 2.000 |
| High resolution limit [Å] | 1.890 | 1.980 |
| Rmerge | 0.382 | |
| Number of reflections | 34696 | |
| <I/σ(I)> | 18.3 | 4.1 |
| Completeness [%] | 95.2 | 83.9 |
| Redundancy | 3.96 | 3.7 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 8.5 | 303 | 35% MMEPEG 2000, 200mM ammonium sulphate, 50mM TrisHCl, 0.2mM PLP, 0.25% DTT, pH 8.5, VAPOR DIFFUSION, HANGING DROP, temperature 303K |
