1Y3S
HIV-1 DIS RNA subtype F- MPD form
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SLS BEAMLINE X06SA |
| Synchrotron site | SLS |
| Beamline | X06SA |
| Temperature [K] | 90 |
| Detector technology | CCD |
| Collection date | 2004-11-05 |
| Detector | MARRESEARCH |
| Wavelength(s) | 0.9196 |
| Spacegroup name | C 2 2 21 |
| Unit cell lengths | 27.243, 117.794, 94.587 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 10.000 - 2.250 |
| R-factor | 0.243 |
| Rwork | 0.243 |
| R-free | 0.26200 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.006 |
| RMSD bond angle | 1.000 |
| Data reduction software | HKL-2000 |
| Data scaling software | SCALEPACK |
| Phasing software | CNS |
| Refinement software | CNS (1.1) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 40.000 | 2.330 |
| High resolution limit [Å] | 2.250 | 2.250 |
| Number of reflections | 13511 | |
| <I/σ(I)> | 30.86 | 4.35 |
| Completeness [%] | 97.9 | 100 |
| Redundancy | 5.7 | 4.7 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 6.5 | 310 | MPD, spermine, KCl, MgCl2, Na Cacodylate, pH 6.5, VAPOR DIFFUSION, SITTING DROP, temperature 310K |
Crystallization Reagents
| ID | crystal ID | solution ID | reagent name | concentration | details |
| 1 | 1 | 1 | MPD | ||
| 10 | 1 | 2 | Na Cacodylate | ||
| 2 | 1 | 1 | spermine | ||
| 3 | 1 | 1 | KCl | ||
| 4 | 1 | 1 | MgCl2 | ||
| 5 | 1 | 1 | Na Cacodylate | ||
| 6 | 1 | 1 | H2O | ||
| 7 | 1 | 2 | MPD | ||
| 8 | 1 | 2 | KCl | ||
| 9 | 1 | 2 | MgCl2 |
