1Y3P
Structure of AlgQ1, alginate-binding protein, complexed with an alginate tetrasaccharide
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SPRING-8 BEAMLINE BL44XU |
| Synchrotron site | SPring-8 |
| Beamline | BL44XU |
| Detector technology | CCD |
| Collection date | 2001-12-03 |
| Detector | OXFORD |
| Wavelength(s) | 0.9 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 58.830, 67.431, 62.957 |
| Unit cell angles | 90.00, 95.90, 90.00 |
Refinement procedure
| Resolution | 14.680 - 2.000 |
| R-factor | 0.2 |
| Rwork | 0.199 |
| R-free | 0.25500 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | AlgQ2 1kwh |
| RMSD bond length | 0.005 |
| RMSD bond angle | 1.200 |
| Data scaling software | d*TREK |
| Phasing software | CNS |
| Refinement software | CNS (1.1) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 14.680 | 2.080 |
| High resolution limit [Å] | 2.000 | 2.000 |
| Number of reflections | 33209 | |
| Completeness [%] | 99.0 | 98.5 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 5.6 | 293 | PEG8000, pH 5.6, VAPOR DIFFUSION, HANGING DROP, temperature 293K |
