1Y3N
Structure of AlgQ1, alginate-binding protein, complexed with an alginate disaccharide
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SPRING-8 BEAMLINE BL44XU |
Synchrotron site | SPring-8 |
Beamline | BL44XU |
Detector technology | CCD |
Collection date | 2002-03-02 |
Detector | OXFORD |
Wavelength(s) | 0.9 |
Spacegroup name | P 1 21 1 |
Unit cell lengths | 58.434, 67.788, 62.811 |
Unit cell angles | 90.00, 95.31, 90.00 |
Refinement procedure
Resolution | 14.910 - 1.600 |
R-factor | 0.197 |
Rwork | 0.197 |
R-free | 0.22400 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | AlgQ2 1kwh |
RMSD bond length | 0.005 |
RMSD bond angle | 1.300 |
Data scaling software | d*TREK |
Phasing software | CNS |
Refinement software | CNS (1.1) |
Data quality characteristics
Overall | |
Low resolution limit [Å] | 40.760 |
High resolution limit [Å] | 1.600 |
Number of reflections | 63420 |
Completeness [%] | 99.5 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 5.6 | 293 | PEG8000, pH 5.6, VAPOR DIFFUSION, HANGING DROP, temperature 293K |