1Y3A
Structure of G-Alpha-I1 bound to a GDP-selective peptide provides insight into guanine nucleotide exchange
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 22-ID |
| Synchrotron site | APS |
| Beamline | 22-ID |
| Temperature [K] | 298 |
| Detector technology | CCD |
| Collection date | 2004-04-03 |
| Detector | MARRESEARCH |
| Wavelength(s) | 1.0093144 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 72.943, 112.785, 109.490 |
| Unit cell angles | 90.00, 93.75, 90.00 |
Refinement procedure
| Resolution | 20.000 - 2.500 |
| R-factor | 0.255 |
| Rwork | 0.240 |
| R-free | 0.28000 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1bof |
| RMSD bond length | 0.008 |
| RMSD bond angle | 1.400 |
| Data reduction software | HKL-2000 |
| Data scaling software | SCALEPACK |
| Phasing software | AMoRE |
| Refinement software | CNS |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 2.590 |
| High resolution limit [Å] | 2.500 | 2.500 |
| Number of reflections | 61384 | |
| <I/σ(I)> | 11.2 | |
| Completeness [%] | 78.2 | 96 |
| Redundancy | 3.5 | 2.7 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 5.5 | 277 | PEG 8000, sucrose, sodium citrate, pH 5.5, VAPOR DIFFUSION, HANGING DROP, temperature 277K |
