1Y39
Co-evolution of protein and RNA structures within a highly conserved ribosomal domain
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SRS BEAMLINE PX9.6 |
| Synchrotron site | SRS |
| Beamline | PX9.6 |
| Temperature [K] | 200 |
| Detector technology | CCD |
| Collection date | 2004-05-08 |
| Detector | ADSC QUANTUM 4 |
| Wavelength(s) | 0.87 |
| Spacegroup name | P 43 21 2 |
| Unit cell lengths | 150.240, 150.240, 62.984 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 18.000 - 2.800 |
| R-factor | 0.22 |
| Rwork | 0.220 |
| R-free | 0.25300 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1hc8 |
| RMSD bond length | 0.006 |
| RMSD bond angle | 1.100 |
| Data reduction software | MOSFLM |
| Data scaling software | CCP4 ((SCALA)) |
| Phasing software | AMoRE |
| Refinement software | CNS (1.0) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 18.000 | 2.900 |
| High resolution limit [Å] | 2.800 | 2.800 |
| Rmerge | 0.061 | 0.445 |
| Number of reflections | 17764 | 1744 |
| <I/σ(I)> | 18 | 5.1 |
| Completeness [%] | 97.5 | 98.1 |
| Redundancy | 4.8 | 4.9 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 6.5 | 310 | PEG600, Magnesium acetate, glycerol, sodium cacodylate, potasium chlroride, pH 6.5, VAPOR DIFFUSION, HANGING DROP, temperature 310K |
Crystallization Reagents
| ID | crystal ID | solution ID | reagent name | concentration | details |
| 1 | 1 | 1 | PEG600 | ||
| 10 | 1 | 2 | potasium chlroride | ||
| 2 | 1 | 1 | Magnesium acetate | ||
| 3 | 1 | 1 | glycerol | ||
| 4 | 1 | 1 | sodium cacodylate | ||
| 5 | 1 | 1 | potasium chlroride | ||
| 6 | 1 | 2 | PEG600 | ||
| 7 | 1 | 2 | Magnesium acetate | ||
| 8 | 1 | 2 | glycerol | ||
| 9 | 1 | 2 | sodium cacodylate |
