1Y2X
Crystal structure of the tetragonal form of the common edible mushroom (Agaricus bisporus) lectin in complex with T-antigen and N-acetylglucosamine
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE ID23-1 |
| Synchrotron site | ESRF |
| Beamline | ID23-1 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2004-07-18 |
| Detector | MARRESEARCH |
| Wavelength(s) | 0.923 |
| Spacegroup name | P 43 21 2 |
| Unit cell lengths | 85.675, 85.675, 257.073 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 30.000 - 2.360 |
| R-factor | 0.22881 |
| Rwork | 0.228 |
| R-free | 0.25241 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | STRUCTURE OF UNLIGANDED LECTIN |
| RMSD bond length | 0.008 |
| RMSD bond angle | 1.215 |
| Data reduction software | MOSFLM |
| Data scaling software | CCP4 ((SCALA)) |
| Phasing software | MOLREP |
| Refinement software | REFMAC (5.1.24) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 30.000 | 2.440 |
| High resolution limit [Å] | 2.350 | 2.360 |
| Number of reflections | 40259 | |
| <I/σ(I)> | 5.5 | 3.5 |
| Completeness [%] | 99.7 | 98.6 |
| Redundancy | 7.9 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 8 | 298 | PEG 8000, lithium sulfate, isopropanol, Tris, pH 8.0, VAPOR DIFFUSION, HANGING DROP, temperature 298K |
