1Y2U
Crystal structure of the common edible mushroom (Agaricus bisporus) lectin in complex with Lacto-N-biose
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | ROTATING ANODE |
Source details | RIGAKU RU300 |
Temperature [K] | 100 |
Detector technology | IMAGE PLATE |
Collection date | 2004-06-23 |
Detector | MARRESEARCH |
Wavelength(s) | 1.5418 |
Spacegroup name | C 2 2 21 |
Unit cell lengths | 91.610, 96.090, 75.060 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 25.000 - 1.850 |
R-factor | 0.2027 |
Rwork | 0.202 |
R-free | 0.21371 |
Structure solution method | FOURIER SYNTHESIS |
RMSD bond length | 0.006 |
RMSD bond angle | 0.948 |
Data reduction software | AUTOMAR |
Refinement software | REFMAC (5.1.24) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 25.000 | 1.900 |
High resolution limit [Å] | 1.800 | 1.800 |
Number of reflections | 28137 | |
<I/σ(I)> | 9.7 | 2.2 |
Completeness [%] | 98.6 | 96 |
Redundancy | 5.4 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 8 | 277 | Sodium formate, Tris, pH 8.0, VAPOR DIFFUSION, HANGING DROP, temperature 277.0K |