1Y2O

Structure of N-terminal domain IRSp53/BAIAP2

Experimental procedure

Experimental method	MAD
Source type	SYNCHROTRON
Source details	SRS BEAMLINE PX14.2
Synchrotron site	SRS
Beamline	PX14.2
Temperature [K]	100
Detector technology	CCD
Collection date	2004-07-25
Detector	ADSC QUANTUM 4
Wavelength(s)	0.9781, 0.9776, 0.9649
Spacegroup name	P 1 21 1
Unit cell lengths	49.879, 64.164, 74.586
Unit cell angles	90.00, 106.18, 90.00

Refinement procedure

Resolution	47.800 - 2.200
R-factor	0.23021
Rwork	0.228
R-free	0.26683
Structure solution method	MAD
RMSD bond length	0.010
RMSD bond angle	1.032
Data reduction software	MOSFLM
Data scaling software	CCP4 ((SCALA))
Phasing software	SOLVE
Refinement software	REFMAC (5.2.0005)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	47.800	2.320
High resolution limit [Å]	2.200	2.200
Rmerge	0.056	0.192
Number of reflections	22459
<I/σ(I)>	25.9	8.4
Completeness [%]	97.4	93.7
Redundancy	3.9	3.7

Crystallization Conditions

crystal ID	method	pH	temperature	details
1