1Y28
Crystal structure of the R220A metBJFIXL HEME domain
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SSRL BEAMLINE BL7-1 |
| Synchrotron site | SSRL |
| Beamline | BL7-1 |
| Temperature [K] | 100 |
| Detector technology | IMAGE PLATE |
| Collection date | 2002-09-01 |
| Detector | MARRESEARCH |
| Wavelength(s) | 1.08 |
| Spacegroup name | H 3 2 |
| Unit cell lengths | 126.761, 126.761, 58.056 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 24.810 - 2.100 |
| R-factor | 0.251 |
| Rwork | 0.251 |
| R-free | 0.28700 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1drm |
| RMSD bond length | 0.007 |
| RMSD bond angle | 1.300 |
| Data reduction software | MOSFLM |
| Data scaling software | CCP4 |
| Phasing software | CNS |
| Refinement software | CNS (1.1) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 24.810 | 2.240 |
| High resolution limit [Å] | 2.100 | 2.100 |
| Rmerge | 0.068 | 0.291 |
| Number of reflections | 10440 | |
| <I/σ(I)> | 5.6 | 7.6 |
| Completeness [%] | 98.7 | 100 |
| Redundancy | 4.1 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7.5 | 277 | 4-4.5M sodium chloride, 5% MPD, pH 7.50, VAPOR DIFFUSION, HANGING DROP, temperature 277K |
