1Y23

Crystal structure of a member of HIT family of proteins from bacillus subtilis

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	NSLS BEAMLINE X4A
Synchrotron site	NSLS
Beamline	X4A
Temperature [K]	100
Detector technology	CCD
Detector	ADSC QUANTUM 4
Wavelength(s)	0.9800
Spacegroup name	P 31 2 1
Unit cell lengths	86.444, 86.444, 180.046
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	19.960 - 2.300
R-factor	0.2131
Rwork	0.213
R-free	0.24900
Structure solution method	SIRAS
Starting model (for MR)	Experimental electron density map from Hg SIRAS density modified phases
RMSD bond length	0.007
RMSD bond angle	1.300
Data reduction software	DENZO
Data scaling software	SCALEPACK
Phasing software	MLPHARE
Refinement software	CNS (1.1)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	19.960	2.380
High resolution limit [Å]	2.300	2.300
Number of reflections	35084
Completeness [%]	99.6	99.8
Redundancy	17.5

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	5.5	291	19.5% Peg 4000, 0.5M Ammonium Acetate, 0.1M Sodium Citrate, pH 5.5, VAPOR DIFFUSION, HANGING DROP, temperature 291K