1Y1U
Structure of unphosphorylated STAT5a
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | BESSY BEAMLINE 14.2 |
| Synchrotron site | BESSY |
| Beamline | 14.2 |
| Temperature [K] | 100 |
| Detector technology | IMAGE PLATE |
| Collection date | 2004-02-18 |
| Detector | MARRESEARCH |
| Wavelength(s) | 0.97 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 143.020, 235.430, 111.420 |
| Unit cell angles | 90.00, 108.76, 90.00 |
Refinement procedure
| Resolution | 78.560 - 3.210 |
| R-factor | 0.26909 |
| Rwork | 0.266 |
| R-free | 0.29891 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1bg1 |
| RMSD bond length | 0.014 |
| RMSD bond angle | 1.685 |
| Data scaling software | XDS |
| Phasing software | PHASER (V. 1.2) |
| Refinement software | REFMAC (5.2.0005) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 90.000 | 3.250 |
| High resolution limit [Å] | 3.210 | 3.210 |
| Rmerge | 0.088 | 0.315 |
| Number of reflections | 56787 | |
| <I/σ(I)> | 9.82 | 2.49 |
| Completeness [%] | 99.9 | 100 |
| Redundancy | 1.98 | 1.99 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 7 | 293 | 1.1M (NH4)2SO4, 20mM MgCl2, 10% glycerol, 50mM HEPES, pH 7.0, VAPOR DIFFUSION, SITTING DROP, temperature 293K |
