1Y1S
Crystal Structure of the Uridine Phosphorylase from Salmonella Typhimurium in Complex with Uracil and Sulfate Ion at 2.55A Resolution
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | MPG/DESY, HAMBURG BEAMLINE BW6 |
| Synchrotron site | MPG/DESY, HAMBURG |
| Beamline | BW6 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2004-06-19 |
| Detector | MARRESEARCH |
| Wavelength(s) | 1.05 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 88.990, 124.120, 133.830 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 26.840 - 2.550 |
| R-factor | 0.189 |
| Rwork | 0.189 |
| R-free | 0.23100 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | SJ9 |
| RMSD bond length | 0.007 |
| RMSD bond angle | 1.500 |
| Data scaling software | XDS |
| Phasing software | MOLREP (V. 7.3) |
| Refinement software | CNS (1.1) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 26.840 | 2.600 |
| High resolution limit [Å] | 2.550 | 2.550 |
| Rmerge | 0.096 | 0.322 |
| Number of reflections | 47348 | |
| <I/σ(I)> | 15.8 | 3.33 |
| Completeness [%] | 96.3 | 95.2 |
| Redundancy | 2.9 | 2.8 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 5.2 | 295 | PEG 4000, SODIUM-ACETATE TRIHYDRATE, uracil, ammonium sulphate, pH 5.2, VAPOR DIFFUSION, HANGING DROP, temperature 295K |
