1Y1R
Crystal Structure of the Uridine Phosphorylase from Salmonella Typhimurium in Complex with Inhibitor and Phosphate Ion at 2.11A Resolution
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | MPG/DESY, HAMBURG BEAMLINE BW6 |
| Synchrotron site | MPG/DESY, HAMBURG |
| Beamline | BW6 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2004-06-19 |
| Detector | MARRESEARCH |
| Wavelength(s) | 1.05 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 88.880, 124.070, 134.400 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 28.750 - 2.110 |
| R-factor | 0.18 |
| Rwork | 0.180 |
| R-free | 0.21900 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1sj9 |
| RMSD bond length | 0.007 |
| RMSD bond angle | 1.500 |
| Data scaling software | XDS |
| Phasing software | MOLREP (V. 7.3) |
| Refinement software | CNS (1.1) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 28.750 | 2.150 |
| High resolution limit [Å] | 2.110 | 2.110 |
| Rmerge | 0.121 | 0.325 |
| Number of reflections | 86174 | |
| <I/σ(I)> | 10.2 | 3.87 |
| Completeness [%] | 97.4 | 90.4 |
| Redundancy | 3.09 | 2.85 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 5.2 | 295 | PEG 4000, SODIUM-ACETATE TRIHYDRATE, 2,2'-Anhydrouridine, sodium phosphate , pH 5.2, VAPOR DIFFUSION, HANGING DROP, temperature 295K |
