1Y1N
Identification of SH3 motif in M. Tuberculosis methionine aminopeptidase suggests a mode of interaction with the ribosome
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ALS BEAMLINE 8.2.2 |
| Synchrotron site | ALS |
| Beamline | 8.2.2 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Detector | ADSC QUANTUM 4 |
| Wavelength(s) | 0.9770 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 49.335, 47.908, 56.819 |
| Unit cell angles | 90.00, 95.11, 90.00 |
Refinement procedure
| Resolution | 20.000 - 1.510 |
| R-factor | 0.213 |
| Rwork | 0.202 |
| R-free | 0.24700 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1c21 |
| RMSD bond length | 0.007 |
| RMSD bond angle | 1.401 |
| Data reduction software | HKL-2000 |
| Data scaling software | SCALEPACK |
| Phasing software | EPMR |
| Refinement software | CNS (1.0) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 20.000 | 1.560 |
| High resolution limit [Å] | 1.510 | 1.510 |
| Rmerge | 0.046 | 0.164 |
| Number of reflections | 41440 | |
| <I/σ(I)> | 30.6 | 7.67 |
| Completeness [%] | 99.5 | 97.1 |
| Redundancy | 26 | 3.4 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION | 6.5 | 273 | Bistris, PEG monomethyl ether 2000, KCl, HEPES, NaCl, pH 6.5, VAPOR DIFFUSION, temperature 273K |
