1Y1A
CRYSTAL STRUCTURE OF CALCIUM AND INTEGRIN BINDING PROTEIN
Experimental procedure
| Experimental method | MAD |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 14-ID-B |
| Synchrotron site | APS |
| Beamline | 14-ID-B |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2004-08-20 |
| Detector | MAR CCD 165 mm |
| Wavelength(s) | 1.53578, 1.53466, 1.47954 |
| Spacegroup name | P 65 2 2 |
| Unit cell lengths | 115.222, 115.222, 268.202 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 47.250 - 2.300 |
| R-factor | 0.236 |
| Rwork | 0.236 |
| R-free | 0.25100 |
| Structure solution method | MAD |
| RMSD bond length | 0.006 |
| RMSD bond angle | 1.200 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | SOLVE |
| Refinement software | CNS (1.1) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 2.380 |
| High resolution limit [Å] | 2.300 | 2.300 |
| Rmerge | 0.067 | 0.388 |
| Number of reflections | 44410 | |
| <I/σ(I)> | 7.9 | 2 |
| Completeness [%] | 92.9 | 65.6 |
| Redundancy | 10.4 | 10.1 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7 | 277 | 20 MG/ML PROTEIN, 50MM HEPES, 3M FORMATE, 300MM NaCl, 1% DMSO, 0.25MM DTT, pH 7.00, VAPOR DIFFUSION, HANGING DROP, temperature 277K |
