1Y18
Fab fragment of catalytic elimination antibody 34E4 E(H50)D mutant in complex with hapten
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ALS BEAMLINE 8.3.1 |
| Synchrotron site | ALS |
| Beamline | 8.3.1 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2003-10-10 |
| Detector | ADSC QUANTUM 210 |
| Wavelength(s) | 1.115870 |
| Spacegroup name | P 32 2 1 |
| Unit cell lengths | 163.000, 163.000, 151.200 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 50.000 - 2.800 |
| R-factor | 0.215 |
| Rwork | 0.215 |
| R-free | 0.25200 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1y0l |
| RMSD bond length | 0.008 |
| RMSD bond angle | 1.400 |
| Data reduction software | HKL-2000 |
| Data scaling software | SCALEPACK |
| Phasing software | AMoRE |
| Refinement software | CNS (1.1) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 2.850 |
| High resolution limit [Å] | 2.800 | 2.800 |
| Number of reflections | 56867 | |
| <I/σ(I)> | 8.2 | 1.5 |
| Completeness [%] | 98.6 | 98.5 |
| Redundancy | 2.4 | 2.4 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 8 | Diammonium hydrogen phosphate, NaCl, imidazole HCl, pH 8.0, VAPOR DIFFUSION, SITTING DROP, temperature 110K |
