1Y17
crystal structure of Aa-X-bp-II, a snake venom protein with the activity of binding to coagulation factor X from Agkistrodon acutus
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SEALED TUBE |
| Source details | RIGAKU |
| Temperature [K] | 298 |
| Detector technology | IMAGE PLATE |
| Detector | MARRESEARCH |
| Wavelength(s) | 1.5418 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 34.944, 53.894, 68.087 |
| Unit cell angles | 90.00, 98.39, 90.00 |
Refinement procedure
| Resolution | 29.080 - 2.400 |
| R-factor | 0.18 |
| Rwork | 0.180 |
| R-free | 0.23600 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.006 |
| RMSD bond angle | 1.200 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | AMoRE |
| Refinement software | CNS (1.0) |
Data quality characteristics
| Outer shell | |
| Low resolution limit [Å] | 2.490 |
| High resolution limit [Å] | 2.400 |
| Completeness [%] | 75 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 4.6 | 298 | PEG MME 550, pH 4.6, VAPOR DIFFUSION, HANGING DROP, temperature 298K |
