1Y11
Mycobacterial adenylyl cyclase Rv1264, holoenzyme, active state
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE ID13 |
| Synchrotron site | ESRF |
| Beamline | ID13 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2003-11-25 |
| Detector | MARRESEARCH |
| Wavelength(s) | 0.9755 |
| Spacegroup name | P 62 2 2 |
| Unit cell lengths | 114.114, 114.114, 151.771 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 25.000 - 3.300 |
| R-factor | 0.23756 |
| Rwork | 0.235 |
| R-free | 0.29941 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | Rv1264 RESIDUES 1-207 (to be deposited) |
| RMSD bond length | 0.011 |
| RMSD bond angle | 1.535 |
| Data reduction software | MOSFLM |
| Data scaling software | CCP4 ((SCALA)) |
| Phasing software | MOLREP |
| Refinement software | REFMAC (5.2.0005) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 30.000 | 3.480 |
| High resolution limit [Å] | 3.300 | 3.300 |
| Rmerge | 0.107 | 0.311 |
| Number of reflections | 8526 | |
| <I/σ(I)> | 6.1 | 2.6 |
| Completeness [%] | 92.5 | 81.2 |
| Redundancy | 2.6 | 2.4 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7.5 | 291 | Lithium sulphate, TRIS, PEG 400, GTP, pH 7.5, VAPOR DIFFUSION, HANGING DROP, temperature 291K |
