1Y0Z

X-ray structure of gene product from Arabidopsis thaliana At3g21360

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 19-BM
Synchrotron site	APS
Beamline	19-BM
Temperature [K]	110
Detector technology	CCD
Collection date	2004-07-31
Detector	APS-1
Wavelength(s)	0.97954
Spacegroup name	C 1 2 1
Unit cell lengths	145.276, 61.094, 114.681
Unit cell angles	90.00, 121.68, 90.00

Refinement procedure

Resolution	43.630 - 2.400
Rwork	0.193
R-free	0.24100
Structure solution method	SAD
RMSD bond length	0.006
RMSD bond angle	1.300
Data reduction software	DENZO
Data scaling software	SCALEPACK
Phasing software	CNS
Refinement software	CNS (1.1)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	43.630	50.000	2.460
High resolution limit [Å]	2.390	5.920	2.400
Rmerge	0.079	0.030	0.283
Total number of observations		2345	2250
Number of reflections	34020
<I/σ(I)>	12.572	4.451	4.451
Completeness [%]	99.8	98.9	99.9
Redundancy	3.6	3.5	3.5

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	7.5	277	10 MG/ML PROTEIN, 16% GLYCEROL, 1.80 M AMMONIUM SULFATE, 0.100 M HEPES, pH 7.5, VAPOR DIFFUSION, HANGING DROP, temperature 277K
2	VAPOR DIFFUSION, HANGING DROP	7.5	277	10 MG/ML PROTEIN, 15% GLYCEROL, 1.75 M AMMONIUM SULFATE, 0.100 M HEPES, pH 7.5, VAPOR DIFFUSION, HANGING DROP, temperature 277K