1Y0P
Flavocytochrome c3 with mesaconate bound
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SRS BEAMLINE PX14.1 |
| Synchrotron site | SRS |
| Beamline | PX14.1 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2003-05-20 |
| Detector | ADSC QUANTUM 4 |
| Wavelength(s) | 0.975 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 45.449, 91.687, 78.287 |
| Unit cell angles | 90.00, 91.13, 90.00 |
Refinement procedure
| Resolution | 24.000 - 1.500 |
| R-factor | 0.15248 |
| Rwork | 0.151 |
| R-free | 0.18353 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1qjd |
| RMSD bond length | 0.015 |
| RMSD bond angle | 1.511 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Refinement software | REFMAC (5.1.24) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 24.000 | 1.550 |
| High resolution limit [Å] | 1.500 | 1.500 |
| Rmerge | 0.048 | 0.373 |
| Number of reflections | 99564 | |
| <I/σ(I)> | 21 | 3.12 |
| Completeness [%] | 97.2 | 94 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 277 | TrisHCl, mesaconate, NaCl, PEG8000, pH 7.8-8.5, VAPOR DIFFUSION, HANGING DROP, temperature 277K |
