1Y0M
Crystal structure of of the SH3 domain of phospholipase C Gamma-1
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | CHESS BEAMLINE F1 |
| Synchrotron site | CHESS |
| Beamline | F1 |
| Temperature [K] | 105 |
| Detector technology | CCD |
| Collection date | 2003-12-16 |
| Detector | ADSC QUANTUM 4 |
| Wavelength(s) | 0.91 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 28.748, 30.978, 29.753 |
| Unit cell angles | 90.00, 92.12, 90.00 |
Refinement procedure
| Resolution | 29.750 - 1.200 |
| Rwork | 0.151 |
| R-free | 0.17700 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.010 |
| RMSD bond angle | 1.291 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | MOLREP |
| Refinement software | REFMAC |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 1.240 |
| High resolution limit [Å] | 1.200 | 1.200 |
| Rmerge | 0.077 | 0.181 |
| Number of reflections | 16059 | |
| Completeness [%] | 96.8 | 79 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7 | 298 | Tacsimate, pH 7.0, VAPOR DIFFUSION, HANGING DROP, temperature 298K |
