1Y0D

T-to-THigh Quaternary Transitions in Human Hemoglobin: desArg141alpha deoxy low-salt

Replaces:  1DSH

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	ROTATING ANODE
Source details	RIGAKU RU200
Temperature [K]	298
Detector technology	AREA DETECTOR
Collection date	1992-10-10
Detector	SDMS
Spacegroup name	P 21 21 2
Unit cell lengths	96.900, 99.000, 65.900
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	10.000 - 2.100
Rwork	0.175
R-free	0.24200
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	1xxt
RMSD bond length	0.011
RMSD bond angle	0.027
Data reduction software	SDMS
Data scaling software	SDMS
Phasing software	X-PLOR
Refinement software	PROLSQ

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	2.270
High resolution limit [Å]	2.100	2.100
Rmerge	0.059	0.189
Number of reflections	36277
<I/σ(I)>	12.2	2.2
Completeness [%]	97.2	86.5
Redundancy	7	3.5

Crystallization Conditions

crystal ID	method	pH	temperature	details
1		7	298	10% PEG 6000, 10 mM potassium phosphate, 100 mM potassium chloride, 3 mM sodium dithionite, 10 mg/ml Hb, pH 7.0, batch, temperature 298K