1XZP

Structure of the GTP-binding protein TrmE from Thermotoga maritima

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ESRF BEAMLINE ID14-1
Synchrotron site	ESRF
Beamline	ID14-1
Temperature [K]	100
Wavelength(s)	0.934
Spacegroup name	P 62
Unit cell lengths	128.855, 128.855, 107.161
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	19.700 - 2.300
R-factor	0.227
Rwork	0.226
R-free	0.25200
Structure solution method	SAD
RMSD bond length	0.006
RMSD bond angle	1.300
Data reduction software	XDS
Data scaling software	XDS
Phasing software	SHARP
Refinement software	CNS

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	19.700	2.400
High resolution limit [Å]	2.300	2.300
Rmerge	0.065	0.440
Number of reflections	44730
<I/σ(I)>	15.5	4.05
Completeness [%]	99.8	99.7
Redundancy	6	6

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	5.5	293	ammonium sulphate, mes, pH 5.5, VAPOR DIFFUSION, HANGING DROP, temperature 293K