1XZI
FUSARIUM SOLANI CUTINASE MUTANT WITH THR 119 REPLACED BY HIS
Experimental procedure
Detector technology | IMAGE PLATE |
Detector | MAR scanner 180 mm plate |
Spacegroup name | P 1 21 1 |
Unit cell lengths | 35.120, 67.360, 37.050 |
Unit cell angles | 90.00, 93.90, 90.00 |
Refinement procedure
Resolution | 6.000 - 1.690 |
R-factor | 0.151 * |
Rwork | 0.148 |
RMSD bond length | 0.009 * |
RMSD bond angle | 1.480 * |
Data reduction software | XDS |
Data scaling software | XDS |
Phasing software | X-PLOR (3.1) |
Refinement software | X-PLOR (3.1) |
Data quality characteristics
Overall | |
High resolution limit [Å] | 1.690 * |
Rmerge | 0.056 |
Total number of observations | 56955 * |
Number of reflections | 18097 |
Completeness [%] | 95.0 |
Redundancy | 3.1 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | Vapor diffusion * |
Crystallization Reagents in Literatures
ID | crystal ID | solution | reagent name | concentration (unit) | details |
1 | 1 | drop | PEG6000 | 2-20 (%(w/v)) | |
2 | 1 | drop | HEPES | 100 (mM) | |
3 | 1 | drop | cutinase | 5-15 (mg/ml) |