1XZD

FUSARIUM SOLANI CUTINASE MUTANT WITH SER 213 REPLACED BY CYS

Experimental procedure

Detector technology	AREA DETECTOR
Detector	SIEMENS-NICOLET X100A
Spacegroup name	P 1 21 1
Unit cell lengths	35.120, 67.360, 37.050
Unit cell angles	90.00, 93.90, 90.00

Refinement procedure

Resolution	8.000 - 2.700
R-factor	0.097
Rwork	0.097
RMSD bond length	0.023
RMSD bond angle	2.900
Data reduction software	XENGEN
Data scaling software	XENGEN
Phasing software	X-PLOR (3.1)
Refinement software	X-PLOR (3.1)

Data quality characteristics

	Overall
High resolution limit [Å]	2.700 *
Rmerge	0.056
Total number of observations	12798 *
Number of reflections	4513
Completeness [%]	88.0 *
Redundancy	2.8

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	Vapor diffusion *

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	drop	PEG6000	2-20 (%(w/v))
2	1	drop	HEPES	100 (mM)
3	1	drop	cutinase	5-15 (mg/ml)