1XZ5
T-to-THigh Quaternary Transitions in Human Hemoglobin: alphaL91A deoxy low-salt
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU RU200 |
| Temperature [K] | 298 |
| Detector technology | AREA DETECTOR |
| Collection date | 1998-11-08 |
| Detector | SDMS |
| Spacegroup name | P 21 21 2 |
| Unit cell lengths | 97.100, 99.000, 65.900 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 10.000 - 2.110 |
| Rwork | 0.176 |
| R-free | 0.24800 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1xxt |
| RMSD bond length | 0.009 |
| RMSD bond angle | 0.022 |
| Data reduction software | SDMS |
| Data scaling software | SDMS |
| Phasing software | X-PLOR |
| Refinement software | PROLSQ |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 2.270 |
| High resolution limit [Å] | 2.110 | 2.110 |
| Rmerge | 0.090 | 0.252 |
| Number of reflections | 36432 | |
| <I/σ(I)> | 7.4 | 1.7 |
| Completeness [%] | 97.6 | 88.2 |
| Redundancy | 6.9 | 3.3 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 7 | 298 | 10% PEG 6000, 10 mM ammonium phosphate, 100 mM potassium chloride, 3 mM sodium dithionite, 10 mg/ml Hb, pH 7.0, batch, temperature 298K |
