1XZ4
Intersubunit Interactions Associated with Tyr42alpha Stabilize the Quaternary-T Tetramer but are not Major Quaternary Constraints in Deoxyhemoglobin: alphaY42A deoxyhemoglobin no-salt
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU RU200 |
| Temperature [K] | 298 |
| Detector technology | AREA DETECTOR |
| Collection date | 1996-09-30 |
| Detector | SDMS |
| Wavelength(s) | 1.5418 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 84.100, 111.900, 64.000 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 10.000 - 2.000 |
| R-factor | 0.18674 |
| Rwork | 0.183 |
| R-free | 0.22372 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1xz2 |
| RMSD bond length | 0.020 |
| RMSD bond angle | 1.890 |
| Data reduction software | SDMS |
| Data scaling software | SDMS |
| Phasing software | X-PLOR |
| Refinement software | REFMAC (5.0) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 2.130 |
| High resolution limit [Å] | 1.980 | 1.980 |
| Rmerge | 0.065 | 0.242 |
| Number of reflections | 41133 | |
| <I/σ(I)> | 9.8 | 1.7 |
| Completeness [%] | 96.7 | 84.1 |
| Redundancy | 6.5 | 2.9 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | batch | 4.6 | 298 | 15% PEG 4000, 50 mM sodium acetate, 100 mM ammonium acetate, 3 mM sodium dithionite, 20 mg/ml Hb, pH 4.6, batch, temperature 298K |
