1XYO
STRUCTURAL COMPARISON OF TWO MAJOR ENDO-1,4-BETA-XYLANASES FROM TRICHODREMA REESEI
Experimental procedure
| Detector technology | IMAGE PLATE |
| Collection date | 1994-07-10 |
| Detector | RIGAKU RAXIS IIC |
| Wavelength(s) | 1.5418 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 81.550, 60.640, 38.250 |
| Unit cell angles | 90.00, 94.46, 90.00 |
Refinement procedure
| Resolution | 8.000 - 1.500 |
| R-factor | 0.19 |
| Rwork | 0.190 |
| RMSD bond length | 0.008 |
| RMSD bond angle | 27.200 * |
| Phasing software | X-PLOR |
| Refinement software | X-PLOR |
Data quality characteristics
| Overall | |
| High resolution limit [Å] | 1.500 * |
| Rmerge | 0.078 |
| Total number of observations | 78692 * |
| Number of reflections | 41141 |
| Completeness [%] | 68.0 |
| Redundancy | 1.9 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | Vapor diffusion, hanging drop * | 4.2 * | Torronen, A., (1993) J. Mol. Biol., 233, 313. * |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | drop | protein | 15 (mg/ml) | |
| 2 | 1 | drop | sodium acetate | 50 (mM) | |
| 3 | 1 | drop | ammonium sulfate | 25 (%) | |
| 4 | 1 | reservoir | ammonium sulfate | 25 (%) |
