1XYN

STRUCTURAL COMPARISON OF TWO MAJOR ENDO-1,4-BETA-XYLANASES FROM TRICHODREMA REESEI

Experimental procedure

Detector technology	IMAGE PLATE
Collection date	1994-06-02
Detector	RIGAKU RAXIS IIC
Wavelength(s)	1.5418
Spacegroup name	C 1 2 1
Unit cell lengths	72.250, 39.010, 54.300
Unit cell angles	90.00, 91.80, 90.00

Refinement procedure

Resolution	8.000 - 2.000
R-factor	0.185
Rwork	0.185
RMSD bond length	0.007
RMSD bond angle	26.800 *
Phasing software	X-PLOR
Refinement software	X-PLOR

Data quality characteristics

	Overall
High resolution limit [Å]	2.000 *
Rmerge	0.090
Total number of observations	20294 *
Number of reflections	7631
Completeness [%]	79.0
Redundancy	2.65

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	Vapor diffusion, hanging drop *	4.5 *

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	drop	protein	10 (mg/ml)
2	1	drop	sodium acetate	50 (mM)
3	1	reservoir	PEG6000	20 (%)
4	1	reservoir	sodium acetate	50 (mM)