1XYF

ENDO-1,4-BETA-XYLANASE FROM STREPTOMYCES OLIVACEOVIRIDIS

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	PHOTON FACTORY BEAMLINE BL-6A
Synchrotron site	Photon Factory
Beamline	BL-6A
Temperature [K]	287
Collection date	1994-12-01
Spacegroup name	P 21 21 21
Unit cell lengths	79.770, 95.010, 141.060
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	8.000 - 1.900
R-factor	0.197 *
Rwork	0.197
R-free	0.23500
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	1xas 2exo
RMSD bond length	0.009
RMSD bond angle	1.500
Data reduction software	DENZO
Data scaling software	SCALEPACK
Phasing software	AMoRE
Refinement software	X-PLOR (3.8)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	100.000	1.971
High resolution limit [Å]	1.900	1.900
Rmerge	0.055	0.254
Total number of observations	441579 *
Number of reflections	80196
<I/σ(I)>	22.4	4.9
Completeness [%]	94.2	77.7
Redundancy	5.6

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	Vapor diffusion, hanging drop *	5.7		25% SATURATED AMMONIUM SULFATE, 2% MCILVAINE BUFFER PH 5.7

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	drop	protein	20 (mg/ml)
2	1	reservoir	ammonium sulfate	25 (%sat)
3	1	reservoir	citric acid	0.1 (M)
4	1	reservoir	sodium phosphate	0.2 (M)