1XY7

X-RAY STRUCTURE OF GENE PRODUCT FROM ARABIDOPSIS THALIANA AT5G48480

Experimental procedure

Experimental method	MAD
Source type	SYNCHROTRON
Source details	APS BEAMLINE 14-ID-B
Synchrotron site	APS
Beamline	14-ID-B
Temperature [K]	100
Detector technology	CCD
Collection date	2004-10-24
Detector	MAR CCD 165 mm
Wavelength(s)	0.97919, 0.97942, 0.95653
Spacegroup name	P 32 2 1
Unit cell lengths	55.871, 55.871, 147.341
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	49.088 - 1.800
R-factor	0.184
Rwork	0.182
R-free	0.22800
Structure solution method	MAD
RMSD bond length	0.019
RMSD bond angle	1.689
Data reduction software	DENZO
Data scaling software	SCALEPACK
Phasing software	CNS
Refinement software	REFMAC (refmac_5.2.0005)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	27.447	50.000	1.840
High resolution limit [Å]	1.800	4.440	1.800
Rmerge	0.037	0.034	0.123
Total number of observations		1680	1614
Number of reflections	25282
<I/σ(I)>	29.85		10.84
Completeness [%]	98.9	88.9	99.6
Redundancy	13.6		10.9

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	5.5	293	12.2 MG/ML REDUCTIVELY METHYLATED PROTEIN, 2.03 M AMMONIUM SULFATE, 0.100 M MES/ACETATE, PH 5.5, VAPOR DIFFUSION, HANGING DROP, TEMPERATURE 293K