1XY0
T-to-THigh Transitions in Human Hemoglobin: alphaK40G deoxy low-salt
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU RU200 |
| Temperature [K] | 298 |
| Detector technology | AREA DETECTOR |
| Collection date | 1995-10-28 |
| Detector | SDMS |
| Spacegroup name | P 21 21 2 |
| Unit cell lengths | 97.200, 99.500, 66.000 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 10.000 - 1.990 |
| Rwork | 0.189 |
| R-free | 0.26000 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1xxt |
| RMSD bond length | 0.012 |
| RMSD bond angle | 0.027 |
| Data reduction software | SDMS |
| Data scaling software | SDMS |
| Phasing software | X-PLOR |
| Refinement software | PROLSQ |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 2.140 | |
| High resolution limit [Å] | 1.880 | 1.880 |
| Rmerge | 0.088 | 0.281 |
| Number of reflections | 43249 | |
| <I/σ(I)> | 7.3 | 1.4 |
| Completeness [%] | 96.8 | 84.1 |
| Redundancy | 6.2 | 3.1 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 7 | 298 | 10% PEG 6000, 10 mM potassium phosphate, 100 mM potassium chloride, 3 mM sodium dithionite, 10 mg/ml Hb, pH 7.0, batch, temperature 298K |
