1XXT
The T-to-T High Transitions in Human Hemoglobin: wild-type deoxy Hb A (low salt, one test set)
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU RU200 |
| Temperature [K] | 298 |
| Detector technology | AREA DETECTOR |
| Collection date | 1990-05-08 |
| Detector | SDMS |
| Spacegroup name | P 21 21 2 |
| Unit cell lengths | 97.000, 99.300, 66.000 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 10.000 - 1.910 |
| Rwork | 0.182 |
| R-free | 0.23900 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1hbb |
| RMSD bond length | 0.010 |
| RMSD bond angle | 0.024 |
| Data reduction software | SDMS |
| Data scaling software | SDMS |
| Phasing software | X-PLOR |
| Refinement software | PROLSQ |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 2.050 |
| High resolution limit [Å] | 1.910 | 1.910 |
| Rmerge | 0.044 | 0.149 |
| Number of reflections | 47999 | |
| <I/σ(I)> | 13.7 | 3 |
| Completeness [%] | 95.3 | 78.1 |
| Redundancy | 6.7 | 2.8 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 7 | 298 | 10% PEG 6000, 10 mM potassium phosphate, 100 mM potassium chloride, 3 mM sodium dithionite, 10 mg/ml hemoglobin, pH 7.0, BATCH, temperature 298K |
