1XXN
Crystal structure of a mesophilic xylanase A from Bacillus subtilis 1A1
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | LNLS BEAMLINE D03B-MX1 |
| Synchrotron site | LNLS |
| Beamline | D03B-MX1 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2004-08-12 |
| Detector | MARRESEARCH |
| Wavelength(s) | 1.427 |
| Spacegroup name | P 21 21 2 |
| Unit cell lengths | 50.931, 70.499, 42.044 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 42.030 - 1.700 |
| R-factor | 0.17344 |
| Rwork | 0.171 |
| R-free | 0.21163 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1hv0 |
| RMSD bond length | 0.013 |
| RMSD bond angle | 1.586 |
| Data scaling software | SCALEPACK |
| Phasing software | AMoRE |
| Refinement software | REFMAC (5.2.0005) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 41.900 | 1.760 |
| High resolution limit [Å] | 1.700 | 1.700 |
| Rmerge | 0.092 | 0.269 |
| Number of reflections | 14449 | |
| <I/σ(I)> | 7.2 | 2.5 |
| Completeness [%] | 81.3 | |
| Redundancy | 1.9 | 1.5 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7.2 | 293 | 0.9M sodium tartrate, pH 7.2, VAPOR DIFFUSION, HANGING DROP, temperature 293K |
