1XXA

C-TERMINAL DOMAIN OF ESCHERICHIA COLI ARGININE REPRESSOR/ L-ARGININE COMPLEX; PB DERIVATIVE

Experimental procedure

Source type	SYNCHROTRON
Source details	NSLS BEAMLINE X25
Synchrotron site	NSLS
Beamline	X25
Detector technology	IMAGE PLATE
Detector	FUJI
Spacegroup name	C 2 2 21
Unit cell lengths	53.500, 83.800, 217.000
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	8.000 - 2.200
R-factor	0.195 *
Rwork	0.200
R-free	0.33000
RMSD bond length	0.010
RMSD bond angle	1.400 *
Data reduction software	DENZO
Data scaling software	SCALEPACK
Phasing software	X-PLOR (3.8)
Refinement software	X-PLOR (3.8)

Data quality characteristics

	Overall
Low resolution limit [Å]	50.000
High resolution limit [Å]	2.200
Rmerge	0.089
Number of reflections	24878
Completeness [%]	95.0
Redundancy	6.2

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	Vapor diffusion, hanging drop *	7.5 *	18 *

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	drop	protein	20 (mg/ml)
2	1	drop	L-arginine	10 (mM)
3	1	drop	NaHepes	50 (mM)
4	1	drop		100 (mM)
5	1	drop		20 (mM)
6	1	drop	MPD	20-25 (%)