1XWY
Crystal structure of tatD deoxyribonuclease from Escherichia coli K12 at 2.0 A resolution
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU RU300 |
| Temperature [K] | 298 |
| Detector technology | IMAGE PLATE |
| Collection date | 2004-09-27 |
| Detector | RIGAKU RAXIS IV |
| Wavelength(s) | 1.5418 |
| Spacegroup name | P 41 |
| Unit cell lengths | 73.885, 73.885, 67.824 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 19.930 - 2.000 |
| R-factor | 0.171 |
| Rwork | 0.171 |
| R-free | 0.21600 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1j6o |
| RMSD bond length | 0.011 |
| RMSD bond angle | 1.500 |
| Data scaling software | SCALEPACK |
| Phasing software | AMoRE |
| Refinement software | CNS (1.1) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 20.000 | 2.070 |
| High resolution limit [Å] | 2.000 | 2.000 |
| Rmerge | 0.071 | 0.447 |
| Number of reflections | 22621 | |
| <I/σ(I)> | 23.5 | 10 |
| Completeness [%] | 91.2 | 84.3 |
| Redundancy | 2.2 | 2 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 7.5 | 279 | 0.1 M Na-HEPES, 1.5 M Lithium Sulfate monohydrate, pH 7.5, VAPOR DIFFUSION, SITTING DROP, temperature 279.0K |
