1XWV
Structure of the house dust mite allergen Der f 2: Implications for function and molecular basis of IgE cross-reactivity
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU RU300 |
| Temperature [K] | 100 |
| Detector technology | IMAGE PLATE |
| Collection date | 2002-12-05 |
| Detector | MAR scanner 345 mm plate |
| Spacegroup name | P 1 |
| Unit cell lengths | 25.712, 47.638, 48.275 |
| Unit cell angles | 85.98, 75.90, 82.76 |
Refinement procedure
| Resolution | 24.430 - 1.830 |
| R-factor | 0.221 |
| Rwork | 0.221 |
| R-free | 0.25700 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1ktj |
| RMSD bond length | 0.008 |
| RMSD bond angle | 1.700 |
| Data reduction software | MOSFLM |
| Data scaling software | SCALA |
| Phasing software | PHASER |
| Refinement software | CNS (1.1) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 24.430 | 1.940 |
| High resolution limit [Å] | 1.830 | 1.830 |
| Rmerge | 0.049 | 0.240 |
| Number of reflections | 18043 | |
| <I/σ(I)> | 9 | 3 |
| Completeness [%] | 93.4 | 85.9 |
| Redundancy | 3.9 | 3.8 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 6 | 293 | PEG 4000, potassium citrate, ammonium acetate, pH 6.0, VAPOR DIFFUSION, HANGING DROP, temperature 293K |
